- Wang Xingtai; Zhuang Hui; Li Hemin et al (National Institute for the Control of Pharmaceutical and Bilolgical Products, Beijing100050)
GBV-C RNA were detected out in the serum of patients with non-A, non - B, non -C, non-D and non -E hepatitis were detected by using RT-PCR. The amplified products were further verified by sequence analysis. The detectablerates in 30 patients and 600 doncrs were6. 6 % and 0. 33%, respectively. It could be speculated that GBV-C might not be the major pathogenic factor for non-A to non-E hepatitis, and a proportion of cases might be asymptomatic carriers.
1996 04 [Abstract][OnlineView][Download 221k] - Pei Jin; Li Zhichao; Bi xiaoying et al (Basic Medical School of Norman Bethune University of Medical Science,Changchun 130021)
IL-2 liposome was prepared by reverse - phase evaporation method. The envelope rate of it was 34.52±1. 12%. Being observed under electron microscope, the IL-2 liposome showed the typical structure of unilaminar vesicles (LUVs) with an average diameter of 0.2μm. The activity of IL-2 liposome was detected by "ConA induced T cell microdetermination". The result showed good stability of IL-2 liposome,and its activity was significantly improved.
1996 04 [Abstract][OnlineView][Download 202k] - Liu Zhonghui; Yang Yu; Yao Yong et al (Basic Medical School of Norman Bethune University of Medical Science,Changchun 130021)
In order to study on the blological activity of follistatin,it was extracted from human follicular fluul and purified by affinity and high performance liquid chromatographies.SDS-PAGE profile showed that the final product was a single band with a molecular weight of 35KDa. Immunological activity detection,activin binding test and FSH secretion inhibition test proved that the final product was a natural biologically-active follistatin and further study on its immunobiology is needed.
1996 04 [Abstract][OnlineView][Download 238k] - Wang Shuhe; Liu Zhonghui et al (Basic Medical School of Norman Bethune University of Medical Science, Changchun 130021)
A lymphocyte proliferatlon inhibition factor was obtained from bovine and porcine thymus by saturated ammonium sulfate precipitation, ultrafiltration and chromatography. The factor, with a molecular weight of about loKDa revealed significant inhibition effect on human T lymphocyte proliferation. Its inhibition rate reached 77. 63±9.37%. However,this factor showed no significant effect on the cytotoxic activity of NK cell.The result indicated that the inhibition was relatively specific.
1996 04 [Abstract][OnlineView][Download 131k] - Huang Xuezhong et al (The 118th Hospital of PLA, Wenzhou 325000)
Prostate specific antigen (PSA) was separated and purified from human seminal plasma by PEG precipitation and Sephadex G - 150 chromatography. After about 80% of non-specific protein was removed by PEG precipitation, PSA was further purified by column chromatography. SDS - PAGE and ELISA proved that the final PSA was immunologically pure.
1996 04 [Abstract][OnlineView][Download 171k]
1996 04 [Abstract][OnlineView][Download 37k] - Zhao Yuewu; Zhao Fenglan; Xu Youmei et al (Research Center for Cancer Biotherapy, Henan Province Hospital, Zhengzhou 450003)
Porin, a major component of outer membrane protein (OMP), was purified from murine typhoid Salmonella by ultrasonication, Triton X - 100 dissolution, hyperfine Sephacryl S - 300 and Sephadex G - 150 gel filtration. It was assayed that the lipopolysaccharide (LPS)content in porin was about 0. 06 %. SDS-PAGE profile showed that porin was a single band with a molecular weight of 36KDa. Western blot demonstrated that porin could specifically combine with rabbit anti-OMPs serum.
1996 04 [Abstract][OnlineView][Download 195k]
1996 04 [Abstract][OnlineView][Download 114k] - Pei Xiaochun; Cui Yumei; Li Qunying et al (Challgchun Institute of Biological Products,Changchun 130062)
In order to study on the immunogenicity of live attenuated hepatitis A vaccine (L-A-1 strain) via oral immunization, the samples of intestinal lavage fluids and serum of 114 mice were collected every week wlthin 1-8 weeks after being immunized and detected for specific antibody response by ELISA. The hepatltis antibody positive conversion rate reached 93. 37% (111/114 ). The peak values of S-IgA positive conversion rate in intestinal lavage fluids were observed at the 1st (65% ) and the 6th (64% )week. Serum IgA was not detected out at the Ist week, however,the positive conversion rate of it reached 100% at the 6th week.The peak value of positive conversion rates of IgM and IgG were observed at the 1st(88% ) and the 3rd (88%) week respectively. By the end of the 8th week, the posltive conversion rates of S-IgA,IgA,IgM and IgG were 40%, 90%, 80% and 80%,respectively. The result showed good immunogenecity of orally administered live attenuated hepatitis vaccine which could successfully stimulate the local and systemic immunosystems of the mice.
1996 04 [Abstract][OnlineView][Download 149k]
1996 04 [Abstract][OnlineView][Download 95k] - Guo Fengyun; Zhang Mei; Wang Yumei et al (Changchun Institute of Biological Products,Changchun 130062)
The serum of patients with bone and joint tuberculosis were detected by anti-PPD ELISA kit. The authors found that the anti - PPD positive rate (90. 79%) was significantly higher than those of the patients with other bone and joint disease and healthy persons.
1996 04 [Abstract][OnlineView][Download 113k] - Chen Shuchang; Liu Peixin; Mei Wei et al (PUMC Hospital, Beijing 100730)
The therapeutic effect of EPO on the CFU-E of 26 cases of MDS in vitro and 13 cases in vivo were observed. The results showed that (1)the CFU - E of patients with MDS were significantly lower than the control; (2) EPO could improve the dose -dependent CFU-E proliferation of the patients. When the concentration of response to EPO was 100u/ml, the number of CFU-E reached the maximum; (3)The effect of EPO on CFU- E was significantly correlated wlth the CFU - E number of the patients. The patient's number of CFU-E more close to the normal value, the more effective response to EPO; (4)EPO was effective in 3 of the 13 cases of MDS. It proved that EPO was effective for part of patients with MDS and could be used as a clinical drug.
1996 04 [Abstract][OnlineView][Download 162k] - Xiao Zhanrong; Hou Qiming; Yang Jingfang et al (National Vaccine & Serum Institute,Beijing 100024)
The children immunized with acellular DTP (DTPa) or whole cell DTP (PTPw) were divided into 3 groups: (1)received the primary immunization of DTPa and a booster of DTPa; (2)received the primary immunization of DTPw and a booster of DTPw;(3)received the primary immunization of DTPw and a booster of DTPa. The serum antibody levels of the children were detected one year after the boosters had been given. The result showed that levels of all kinds of antibodies were significantly decreased in comparision with those detected one month after the boosters were given. The anti -FHA of the 3 groups were above protective level (20 EU/ml ), however, only the anti - PT of the group (1) was above 20EU/ml. The pertussis agglutlnating antibody levels of most of the childen were less than 1: 320,but the anti-DT and anti-TT of them were above protective levels.
1996 04 [Abstract][OnlineView][Download 170k]
1996 04 [Abstract][OnlineView][Download 267k] -
1996 04 [Abstract][OnlineView][Download 236k]
1996 04 [Abstract][OnlineView][Download 42k] -
1996 04 [Abstract][OnlineView][Download 73k] 下载本期数据