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1988 00 [Abstract][OnlineView][Download 37K]
1988 00 [Abstract][OnlineView][Download 221K] -
1988 00 [Abstract][OnlineView][Download 41K] - Wang Changyu Wang Guiqiu Wang Yongji (National Vaccine and Serum Institute,Beijing)
The DNAs of seven varicella-zoster virus(VZV)isolates were extraeted by disrup- tion of the purified viral nueleocapsids.The VZV DNAs were cleaved by two restriction end- onueleases.and analysed by agarose gel e’e trophores s.In the gel pattern of VZV DNAs cleaved with EcoRI,variations appeared among EcoRI-G,EcoRI-J and EcoRI-p fragments.The here- rogeneity in the gel pattern of VZV DNAs cleaved with BamHI was shown mainly in fragments with low molecular weight.The comprehensive compatison of the gel patterns of the tested VZV DNAs indicated that the seven isolates were non-homologous.
1988 00 [Abstract][OnlineView][Download 168K] - Xu Deqi Dong Wei Yu Wanlong Jia GuoFu (Changchun Institute of Biological Products,Ministry of Public Health)
By Using 32P—labeled whole genomic DNA of HBV ayw as a probe,the samples collected from partial C011ection of surose rate centrifugation during the preparation of HBV Plasma vace- ine and collected randomly samples from final bulk were analysed.The result showed by Mole- cular hybridization that all of the samples containing whethef long tubular form particles or short tubular form particles obseved under electromicroscope are negative,but the samples con- taining Danes’particles form 3 lots of the preparation including 6 collections are positive.This result indicates that the presence of long tubular form Particles evaluated by electromicroscope as samples non-passed needs to be furtller discussion.The sensitivity of the HBV DNA probe call reach 1pg.
1988 00 [Abstract][OnlineView][Download 694K] - Song Shuzhen Chen Jinrong Zeng Lun Wang Bingrui (Lanzhou Institute of Biological Products,Ministry of Public Health)
A large plasmid was discovered in a rough type strain of Sh.sonnei named as S_7R.The presence of the large plasmid was reported only in S form of Sh.sonnei strains, which was considered relating to the I form antigen before. The plasmld is about 64 Md in size and 56 Md smaller than I form large plasmid(120Md)in wild type,which is thought to be the deletion mutant of the wild type plasmid and was iden- tified and confirmed by experiments in our 1aboratory.It was shown that the gene regions encoding 1virulence and I form antigen were deleted from the original plasmid by about 56Md in 1ength. The large plasid in the parent S_7 strain of the S_7R type is different from S_7R and the Wild strain.It is 10Md bigger than S_7R and 46Md smaller than the wild one.It encodes I form alltigen efficiently but shows no virulence.Therefore S_7 strain cohld be used as a donor to cons- trut I form antigen-containing dysentery vaccine safely. The discovery of the large ptasmid in S_7R would be benefit to the genetical study of I form large plasmid and I form antigen.
1988 00 [Abstract][OnlineView][Download 683K] -
1988 00 [Abstract][OnlineView][Download 47K] - Ding Shaoqing Ye Renbang Yuan Zenglin (National Institute for the Control of Pharmaceutical and Biological Products,National Center for Medical Culture Collection,Beijing)
712 clinical isolates of S pneumoniae was serogrouped into 42 groups or Lypes by“quel- lung”reaction with lyping sera among which types 40,42,44 and 47 had not been found in Chlna before.of the 712 strains,type(group)5,6,1,19,23,2,14 and 3 were the most frequently encountered ones and these eight types(groups)totaled 453 strains(63.3%)and type 16A and 10C are found as new types. of the 180 isolates ftom CSF,137 strains(76.1%)of type(group)2,5,6,1,27,23,14,12 and 7 were the common estones;52 of 71 strains from blood(72.6%)the most common types(groups) were 1,5,6,14,19,7,8 and 35,183 of 253 strains(72.3%)isolated from otitis fluids.The most common types(groups)were 19,6,5,23,14,3,1 and 15.
1988 00 [Abstract][OnlineView][Download 202K] -
1988 00 [Abstract][OnlineView][Download 39K] - Song Yiming Huang Peiren Tong Kuitang (Shanghai Institute of Biological Products,Ministry of Public Health)
Restriction enzyme map of hunman IFN-α_1 gene in recombinant E.coli has been identified after high density fermentation.The resufts ind:cate that human IFN-α_1 gene has one EcoRI,one BglⅡ,two Pvu Ⅱ,four Sau3A and Alu Ⅰsites which are in conformity with the date reported in literatures.
1988 00 [Abstract][OnlineView][Download 418K] - Dai Yaoxun Wang Canzhen Huang Peiren Tong Kuitang (Shanghai Institute of Biological Products,Ministry of Public Health)
In order to veelfy the Subtype of IFN-α-1 structural gene in engineeing strain BMH_(71)-18/ pBV857 and to monitor any change of the gene that might happen after felmentation,we isolate its structural gene form harvestied culture via high density fermentation and then sequence it by the Sanger dideoxynucleotide chain termination method and by the Ray method based on adding separately synthesised primers.In eomparisoh of this Sequence with the sequece of original strain and the sequence reported in 1iterature,we did not find any change of base pair.
1988 00 [Abstract][OnlineView][Download 79K] - Shi Liangru (Wuhan Institute of Biological Products,Ministry of Public Health)
The results of characterizattion of Wuhan(Wu)series monoclconal antibedies(MeAbs) against human T lymphocytes and its subsets in accordance with the requirement of Third International Human Leucocyte Differentiation Antigens Workshop Were reported.Wu series McAbs corresponded to OKT series consisted of WuT1(OKT1,CD5);WuT3(OKT3,CD3);WuT4 (OKT4,CD4);WuT6,(OKT,CD_1a);WuT8(OKT8,CD8):WuT0(OKT9,anti TfR);WuT10(OKT10, CD38);WuT11(OKT11,CD2)and WuTac(Tac,CD25)etc.
1988 00 [Abstract][OnlineView][Download 226K] - Zhao Mei Luo Guoxiang Xu Jingyun Song Ping Chen Shufan (Institute of Medical Biology,Chinese Academy of Medical Sciences,Kunming)
A virological survey of 17 viruses and/or antibodies in rhesus monkeys were carried out This repors presents data based on 16,321 necropsies,including 6,715 virus isolations,8,932 neurohistologlcal tests,and 7,379 serologlogcal surveys.SFV,SV40 and their antibodles were. demonstrated most frequently,the next most frequent were antibodies to measles and vaccinia viruses.No B viras was found,but there were 12.89% of antibodies to HSV-1.This should be carefully evaluated.
1988 00 [Abstract][OnlineView][Download 170K] - Zhao Shuliang (Cheugdu Institute of Biological Products,Ministry of Public Health)
After 2-3 muscular iniections with adsorbed tetanus toxoid as basic immunjzation, the donors were further hyperimmunized with a big dose(36Lf/0.5ml)of thepurfied reranus toxoid.Accordinly,a plenty amount of immune plasma with high antibody titer was determined with ID techn que in vitro. From above pooling plasma we prepared three barches of TIG.The antibody titer of TIG was 260-330IU/ml which was concentrated 13-18 times compared witl poeling plasma.The stability of TIG was desirable as well. In first stage of clinical trial for 20 adults with 250IU/m1 TIG,all the persons have shown satisfactory startding to TIG without any Io al and all over rea tions.For second stage, the same dosage of TIG Was intected to 156 cases snffering from different open tratma.The resnlts has given significant evidence for prevention tetams with TIG.The level of antibodies in ser also indicated that TIG is better than TAT. Our results have demonstrated that TIG was effective,reliable and very safe for not only prevention but also therapy of tetanus disease.
1988 00 [Abstract][OnlineView][Download 202K] -
1988 00 [Abstract][OnlineView][Download 51K] - Yu Wanlong (Changchun Institute of Biologicol Products,Ministry of Public Health)
282 HBV seronegative children aged 8-11 were ineluded in the trial.151 children were vaccinated with 10μg of HBsAg in one dose on month 0,1,2.Other 131 children Were immunized on month 0,1,6.Former anti-HBs seroconvers ons rate at 3 and 36 months were 96.5% and 83.7%,respertively.This result showed that about 12.8% vaceine responders have been negative,while later no changed for 24 months,In anti-HBs level,ant-HBs peak for former was found at 6 months after intial vaccination,but at 7-12 months for later.Antiody level at 24 months was 3.5 times of former. Formet anti-HBs at 12 months decreased 4.3 times from that at 6 months.After 24 months ant-HBs 1evel decreased slowly.Ant-HBs value at 36 months was still maintained over 70.3 mIU.The results support that the persistence of anti-HBs after the Vaccination at least is over 3 years.
1988 00 [Abstract][OnlineView][Download 90K] - Zhu Tingyung (Sanitation and Antiepidemic Station of Chengjiang County,Yunnan)
The author observed the lasting time of placenta transferng measles antibody in infants.The titre of placenta transfering antibody began to drop month by month from the fourth month after birth.In the eighth month,the antibody couldn’t be detected in 93.33% of the infants.The month on which the first immunization of live measles vaccine should be taken according to the results of study and the conditions of our country were discussed.
1988 00 [Abstract][OnlineView][Download 79K]
1988 00 [Abstract][OnlineView][Download 191K] -
1988 00 [Abstract][OnlineView][Download 49K] 下载本期数据