- Zhang Ling Hubei Provincial Anti-epidemic Centre,Wuhan
The study of sedondary immunization in vitro was carried out by cultured the primarily immunized mouse spleen cell with antigen,i.e.CEM cell treated by cold alcohol. The results showed that in the presence of PWM and TCM,B cell could secret the specific antibody against the antigen.
1991 04 [Abstract][OnlineView][Download 98K] - Dai Changbai Institute of Medical Biology,Chinese Academy of Medical Sciences,Kunming
Two recombinant vaccinia virus(RV-1 and RV-2)strains containing a full genome of polio virus(PV)type Ⅰ and encoding structural protein Vpl 2.5 Kd cDNA of PV have successfully expressed the antigenic protein of PV type Ⅰ.The antigen was located in the infected Hep-2 cell with an appearence specific granular particles by IF assay.The expressed PV proteins showed as four bands in RV-1 and two bands in RV-2 on Western Blot.They reacted well with anti-PV McAb.The animals immunlized with the two recombinant virus strain could produce neutralization antibodies against PV type Ⅰ.This paper also presented the preliminary results of expression level of PV type Ⅰ anti- gen of recombinant virus
1991 04 [Abstract][OnlineView][Download 392K] -
1991 04 [Abstract][OnlineView][Download 36K] - Sun Maosneng Institute of Medical Biology,Chinese Academy of Medical Sciences,Kunming
Two animal rotaviruse strains,Simian rotavirus SA11(serotype 3)and calf rotavirus NCDV(serotype 6)were pass,aged on BGMK cell(a africa green monkey kideny cell line)at 36℃,33℃ and 29℃ respectively Hemoagglutinin activities of the rotaviruses passaged at lower temperature decreased obviously.Infectivities of rotaviruses passaged at lower temperature in cell culture at 39℃ were lower than those of the original strains. The results of sero-neutralization test showed that after passages through 50 times,the two strains could still be neutralized by antibody to original strains.PAGE analysis indicated that SA11 remained their genetic stability throughout the study,while the fifth segment of the genome RNA of NCDV was changed.
1991 04 [Abstract][OnlineView][Download 145K] -
1991 04 [Abstract][OnlineView][Download 42K] - Zhang Quanyi Changchun Institute of Biological Products,Changchun
BALB/C mice were immunized with the M_5-type associated antigen of acute non-lymphocytic leukemia,The spleen cells were fused with NS-1/1.Aq.4.1 m yeloma cells, and 2 cell strains which could secret anti-acute non-lymphocytic leukemia M_5-type monocl- onal antibodies were established.All the monoclonal antibodies secreted by these cell stra- ins gave positive reaction with the leukemia cells from acute non-lymphocytic leukemia M_2- type patient,but could not give any reaction with the cells from other type of leukemia patients.Themonoclonal antibodies produced by the cell strains showed good specificity.The result of CFu-GM culture did not affect myeloid stem cells.
1991 04 [Abstract][OnlineView][Download 280K] - Tan Xiangyang Wuhan Institute of Biological Products.Wuhan
By using the techniques of immunohistological enzyme staining and immunoc- ytological fluorescent staining,the reactivities of WuT 3(anti-CD3),WuT4(anti-CD4), WuT8(anti-CD8),WuT9(anti-CD71 or TfR)and WuTac(anti-CD25 or IL-2R)MAbs with 25 normal human tissues in vitro were detected.The results showed that medullary th- ymocytes,the T cell area cells in lymph nods e and tonsil,the arterial lymphatic sheath cells in spl- een and the scattered Iymphocytes and lymph nodule cells in gastrointestinal mucosa were stain- ed with WuT 3,A few scattered cells in germinal center and mantle zone were also stained with WuT3.Cortical thymocyes and some of medullary thymacytes were stained with WuT 4 and WuT8.The numbers of WuT 8 positive mudullary thymocytes were much fewer than that of WuT4.The distribution of WuT 4 and WuT 8 positive Iymphocytes in peripheral lymphoid'tissues were simolar to that of WuT3,but with fewer numbers.When sta- ined with WuT9,26% of bone marrow cells,a few scattered cortical thymocytes and the bottom cells of intestinal mucosa glands were positive.WuTac stained with only a few medullary thym- ocytes.All of the above 5 MAbs for therapeutical use basically showed negative reactions with other non lymphotic tissues and cells.
1991 04 [Abstract][OnlineView][Download 202K]
1991 04 [Abstract][OnlineView][Download 50K] - Yin Hongzhang National Vaccine and Sera Institute,Beijing
Polyclonal antibodies from mouse ascites can be produced on the basis routine immunization of BALB/c mice and the injection of the mouse tumor cell line(Sp2/0).5 to 10 ml ascites can be obtained from each mouse.The yield by volume is 10—20 fold in comparison with the antiserum.The ELISA titer of the ascitic Abs activity(TA=ELISA titer X volume of ascites or sera)is 5—10 times higher than that of serum Abs activity.It is a new way to produ- ce mouse anti-human-globulin as a seeond Abs used in mouse McAb immunoassy without cross reactivity caused by other second anti-bodies(such as goat anti-human-globulin)and this method can also be applied to prepare other specific Abs in reasonable amount.
1991 04 [Abstract][OnlineView][Download 114K]
1991 04 [Abstract][OnlineView][Download 30K] - Gao Enming National Institute for the Control of Pharmaceutical and Biological Products,Beijing
Reference strains of W group Gonococcus from Center for Medical Culture Colle- ction(CMCC)were used in this study.Rabbits were immunized according to Sandstrom method. ELISA was performed to detect the antibodies in sera collected befre immunization,and after immunizing with adjuvant,antigens,and boost.Results showed that andibodies elevated gradually.The highest ELISA titre among the sera obtained reached 1:5120-1:20480.Both formalin-fixed whole cell suspension and lawn agglomerate showed only weak reaction with antisera,but heated whole cell suspesion(after boiling forl hr in a water bath)agglomerated with antisera strongly.
1991 04 [Abstract][OnlineView][Download 132K] - Li Zhengzhi Chengdu Institute of Biological Products,Chengdu
An acellular vaccine was made by extracting the antigen from the slime of Pseud- domonas aeruginosa strains.This vaccine consisted mainly of proteins,Polysaccharides and nucleic acid.The advantages of this vaccine were low toxicity,low immune doses(40μg/ per mouse)and production of high antibody titer(1:2560PHA).The extraction procedures were easy to perform and suitable for larger scale production.
1991 04 [Abstract][OnlineView][Download 115K] - Ning Yang National Vaccine and Serum Institute,Beijing
The SRBC was coated with rabbit antimycoplasma IgG and was used to identify the isolated mycoplasma and standard mycoplasma cultures.It was demonstrated that the RPHA was sensitive,specific and easy to judge between negative and positive results. The assay is stable and reproducible,and is a simple,rapid,economic method for myco- plasma identification.
1991 04 [Abstract][OnlineView][Download 111K] - Feng DuoJia Cheng Yi Lanzhou Institute of Biological Products,Lanzhou
A chromogenic LAL assay(CLA)was developed and studied for the quantitat- tion of endotoxin in biological products.Depending upon the determination of the range of rabbit pyrogen dose (RPD),the standard of judgement was established as following: if the endotoxin concentration was lower than 1.66IU/ml,the product is judged as gualified;if higher than 5.23IU/ml,it is judged as unqualified;if between1.66-and5.23IU/ ml,it should be arbitrated by rabbit test.By testing 10kinds of biological products,no interference factor was found except plasma,ACD and sodium citrate(SC).This interference could be eliminated or inhibited by dilution,adding Mg~(++)or in combination with hea- ting.By comparing the results of both CLA and rabbit test from 10 kinds of biological pro- ducts(534 batches),good coincidence was obtained in 95.45% of total samples tested, and only 9.55% samples required to be further arbitrated by rabbit test.We found that the CLA is specific,sensitive,rapid and reproducible.Moreove,the Azodye method of CLA seems to be more accurate than pNA method for routine test.
1991 04 [Abstract][OnlineView][Download 222K] -
1991 04 [Abstract][OnlineView][Download 41K] - Wang Pengfu Changchun Institute of Biological Products,Changchun
On the basis of satisfactory results in limited numbers of recipients(30volunt- eers),we observed an enlarged group of 350 healthy school children from age 8—11,who were randomly divided into 5 groups and injected with different doses,and titres by fiffer- ent routes with the experimental hepatitis A vaccine.The results showed that none of the recipients developed any local or systemic adverse reaction,and no abnormal elevation of body temperature or liver enzyme were found.The seroconversion rates at four weeks after immunization ranged from 70—100%,and 98—100% at 8 weeks.It suggested that the exper- imental vaccine was safe with good immunogenicity.
1991 04 [Abstract][OnlineView][Download 131K] - Yu Wanlong Changchun Institute of Biological Products,Changchun
A total of 457 school children were observed for 4—5 years after vaccina- ting with HB vaccine.151 of the 457 children were immunized with 10μg dose at months 0,1,2 and observed for 5 years.The seroconversion rate of the antibody was 72%.Antibody level was 40.5 mIU/ml.After booster immunization,the seroconversi on rate rose to 97.3% and antibody level rose to 548.8mIU/ml.The other 306 chi- ldren were immnunized at 0,1,6 month with 5,10,20μg of vaccine respectively,and ob- served for 4years.The seroconversion rates were 82%,100% and 94.2% respectively.This result indicated that the immunization schedule at 0,1,6month was better than that at 0,1,2 month.We also observed the higher antibody titre,the slower reduction of antibo- dy and the longer duration of anti—HBs antibody.In the group of 0,1,2 month immuniza- tion schedule,infective rate of HBV was 6.8% and protective rate was 86.4% during the 5- year observation.However,those immunized at 0,1,6 months,no one developed HBV infec- tion during the 4 years period. This observation revealed that the antibody could be maintained at least for a peri- od of 4years for both two immunization schedules and that the booster vaccination after 5 years was feasible.
1991 04 [Abstract][OnlineView][Download 159K]
1991 04 [Abstract][OnlineView][Download 274K] -
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