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1991 02 [Abstract][OnlineView][Download 37K]
1991 02 [Abstract][OnlineView][Download 240K] - Lin Xue-yan Wuhan Institute of Biological Products
The monoclonal antibody 3Al,which is specific for CD_7 antigen deter-minant,was labeled with ~(125)I.The ~(125)I-3A_1 immunoconjugate was injected into Tly-mphoma (CEM) bearing nude mice,26 hours later,immunoscintigraphy was performedusing a SPECT machine.These nude mice were sacrificed 96 hours after injection andin vivo distribution of ~(125)I-3A_1 was determined using r counter.The result shows thatthe imrnunoconjugate ~(125)I-3A_1 mainly concentrated,in tumor area in CEM tumor-bea-ring nude mice.No obvious concentration of ~(125)I-3A_1 was founded in nasopharyngeal ca-rcinoma bearing nude mice.This work offers the possibility for clinical application ofMcAb 3 A_1 in the diagnosis and therapy of T-lineage lymphoma.
1991 02 [Abstract][OnlineView][Download 240K] - Lai Jianping Wuhan Institute for Biological Products
Using the technique of immune-spotting with pre-S_1 and pre-S_2 monoc-lonal antibodies,the pre-S_1 and pre-S_2 proteins were investigated in the same bulkof plasma-derived hepatitis B vaccine antigen prepared by two different methods-2 stepheating inactivation and 3-step chemical inactivation respectively.The results revea-led that heat inactivation could preservo pre-S_1 and pre-S_2 proteins,while 3-step ch-emical inactivation destroy them.These results implied that the plasma-derived HB va-ccine prepared by heat inactivation retained almost the same antigenic components asnaturally occured.This paper reported the preparation of hepatitis B vaccine containing both pre-S_1and pre-S_2 proteins in addition to S protein and their potential functions were discu-ssed.
1991 02 [Abstract][OnlineView][Download 199K] - Gu Zhengxian National Vaccine and Serum Institute,Beijing
Three monoclonal anti-keratin antibodies which can be used in tumor cl-assification have been obtained.HK_2 and K_(174) can recognize both higher and lowermolecular weight keratins,and reacts not only with squamous epithelial cells and sq-uamous cell carcinomas,but also with simple epithelial cells and adenocarcinomas byimmunohistochemical staining.However,HK_2 showed better reaction with carcinomasoriginated from simple and gland epithelial cells than K_(174).K_(174) reacted more stronglywith squamous cell carcinomas than HK_2.The positive rate of low-differentiated andundifferentiated carcinomas stained with mixed HK_2 and K_(174) was 87.1%.K_(27) mainly re-cognized higher molecular weight keratins,and thus only reacted with superbasal la-yers of squamous epithelial and squamous cell carcinomas.However,the basal layer ofsquamous epithelium,simple epithelium and adenocarcinomas (with exception of lungadenocarcinomas) were all negatively stained with K_(27).Thus,K_(27) is useful for diffe-rentiating squamous cell carcinomas and adenocarcinoma,except lung tumor.
1991 02 [Abstract][OnlineView][Download 563K] - Tang Enhua Institute of Medical Biology,Chinese Aeademy of Medical Sciences,Kunming
23 of 34 monoclonal antibodies (McAbs) to poliovirus have been scre-ened for making two panels of diagnostic kit.One panel made of 3 fluorescence-lablled McAbs was used for pathogenically identifying poliovirus.With the otherpanel it was possible to characterize the viruses,identify the intertypic different-iation of poliovirus strains,analyse the antigenic variation and recognize the va-ccine-related strains by immunfluorescence assay or microneutralization assay.In recent years,applying above methods,we have investigated 286 Poliovirusesstrains.The results have shown that the methods were specific,simple,rapid andsensitive for the diagnosis of polioviruses.The McAbs were excellent for analyz-ing topological relationships of antigenic epitopes and recognizing the vaccine-re-lated and/or wild strains.
1991 02 [Abstract][OnlineView][Download 212K] - Liu Dezheng National Vaccine & Serum Institute,Beijing
B.Pertussis phase I was cultivated in SS medium supplemented with 0.1%Heptakis (dimethyl-b-cyclodextrin) in large shaking bottoles.From the culture superna-tant,two kinds of main protective antigens FHA and PT were extracted by precipita-tion with ammonium sulfate and endotoxin was removed by density gradient centrifug-ation.The FHA and PT preparation was detoxified in an appropriate degree with glu-taraldehyde.Finally the acellular pertussis vaccine (ADPT) was obtained.The test showed that ADPT vaccine did not cause the fever in rabbits and ind-uce leucocytosis and histamin sensitizing activity inmice significantly.The vaccineprepared by the method mention-above conferrd good protection and higher antib-ody response to B.pertussis in mice.Toxicity reversion of the vaccine was not fo-und when stored at 37℃ for 3 months.
1991 02 [Abstract][OnlineView][Download 150K]
1991 02 [Abstract][OnlineView][Download 42K] - Zeng Guohua Changchun Institute of Biological Products
Chen Yikai J.Shevitz New Brunswick Scientific Co.,Edison.N.J.USA
Hybridoma cell line 15B_4 producing antibody against human blood group Awas cultured with cell culture perfusion in the bioreactor (Celligen).Cell density re-ached 1.6×10~7/ml and the amount of culture medium was increased from 1000ml to2300ml each day.The medium was reduced to.1000ml after 14th day,then the amountdead cells were increased with the decrease of perfusion quantity.Hemagglutinating ti-ter was greater to 2~(14) comparable with the antibody titer of ascites:The IgM yieldwas 1.6-1.86g/L.Metabolism of 15B_4 cells was related to concentration of glucoseand lactic acid,but not ammonia.The celligen was suitable for cell culture of hybridoma in high density,When sti-rring rate reached 150r/min,the cells were disrupted by shearing force.The dissolvedoxygen control system remains to be improved.
1991 02 [Abstract][OnlineView][Download 140K] - Yu Yongping National Institute for the Control of Pharmaceutical and Biological Products,Beijing
According to the program of international collaborative study on de-fining If-units for diphtheria and tetanus toxoids as international reference materi-als,we have calibrated the two proposed international reference toxoids (DIET andTEFT) by Ramon test.The results obtained from our laboratory were close to thoseobtsined from international collaborative assay by 13 laboratories.A number of diphtheria and tetanus toxoids were titrated both by Levine-Wy-man flocculation and rocket immunoelectrophoresis.These results were compared withthose obtained by Ramon test.It was demonstrated that the rocket immunoelectro-phoresis could be used as an alternative to the flocculation test.
1991 02 [Abstract][OnlineView][Download 115K] -
1991 02 [Abstract][OnlineView][Download 632K]
1991 02 [Abstract][OnlineView][Download 46K] -
1991 02 [Abstract][OnlineView][Download 75K] - Zhu Meifen Shanghai Institute of Biological Products,Shanghai
The bacteriophage method was adopted for the detection of Ps.aeruginosain the stool of laboratory animals.Ps.aeruginosa was found in 148 (32.67%) of 453stool samples;17 (22.1%) out of 148 are from 77 samples of SPF and CL mice;79(38.4%)from 206 samples of CV mice;9 (45%) from 20 samples of CV rabbit;13 (17.8%) from73 samples of CV guinea pig;30 (39%) from 77 samples of CV hamster.As comparedwith the result of culture method,the detectable rate of the latter was 12/453 (2.65%).The result showed that the bacteriophage method is simple and effective,and has theadvantage of high specificity and detectable rate.
1991 02 [Abstract][OnlineView][Download 113K] -
1991 02 [Abstract][OnlineView][Download 105K]
1991 02 [Abstract][OnlineView][Download 73K] -
1991 02 [Abstract][OnlineView][Download 44K]
1991 02 [Abstract][OnlineView][Download 101K] -
1991 02 [Abstract][OnlineView][Download 51K]
1991 02 [Abstract][OnlineView][Download 43K] -
1991 02 [Abstract][OnlineView][Download 43K] 下载本期数据