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1991 01 [Abstract][OnlineView][Download 267K] - Shen Yulin et al Lanzhou Institute of Biological Products, Lanzhou
It was shown that the typhoid Vi capsular pol yssacharide vaccine purified with undenatured method was not only antigenic but also protective against the typhoid fever. We have tried to purify the Vi antigen using the procedure reported by Robbins et al with some modifications and tested the chemical and biological properties of it. The rsults were agreeable with the report by above mentioned authors. It showed that one does of 0.5～1.0μg typhoid Vi antigen could produce a high protection effect against the challenge with virulent salmonella typhi strain Ty2 in mice.
1991 01 [Abstract][OnlineView][Download 158K] - Lu Zhongyun Zhou Bei ping Wuhan Institute of Biological Products, Wuhan
This paper presents a method for preparation of small, active fragments from mouse monoclonal IgM antibodies. We have compared the influence of temperature on the splitting products at 37℃ and 4℃ with trypsin digestion. The small fragments were alkylated with i odoacetamide and purified by means of Sephacryl S-200 column. The fragments obtained at 37℃ had a molecular weight approximately 50000(Fab) as determined by PAGE; its immunoactivity was lower. But at 4℃, the molecular weights of the fragments ranged from 80000 to 110000 (Fab μ),and its immunoactivity was similar to the original IgM. The Fab μ fragments were labelled with horseradish peroxidase (HRP) and these conjugtes have been used in sandwich-ELISA with satisfactory results.
1991 01 [Abstract][OnlineView][Download 299K]
1991 01 [Abstract][OnlineView][Download 47K] - Wang Shipeng et al Chengdu Institute of Biological Products, Chengdu
This paper reported that three batches of the sera prepared based on the supplemented IATS-20 were examined in this laboratory and 10 laboratories from other 4 countries. After the quality of the sera successfully had been passed by inspection of NICPBP of China, many sets of them were sent to the laboratories of other countries for further investigation. The results from 10 laboratories of 4 countries showed the sera were specific and sensitive in slide agglutination, and 97.51 percent of 1685 clinical isolates were typable. However, 3.62 percent of these strai ns, especially in Pakistan and U. S. A. (9.69 and 7.95 percent respectively) belonged to 3 new supplemented serotypes. Obviously it would be better if 3 new antigens could be added to IATS.
1991 01 [Abstract][OnlineView][Download 174K] - Nie Zilin et al National Institute for the Control of Pharmaceutical and Biological Products, Beijing
3 virus strains such as Z_(10). HB_(55) and L_(99) were selected to study the passage adaption of hemorrhagic fever with renal syndrome virus (HFRSV) in Vero cell and 3 batches of inactivated vaccine were developed by these 3 virus strains. Rabbits were immunized with the 3 batches of vaccine. The results showed that the neutralizing antibody titres of the rabbit immune sera was 100% at 4 weeks after 2 times of immunization. The preliminary results indicated that the Vero cell line can be used for production of HFRS inactivated vaccine.
1991 01 [Abstract][OnlineView][Download 165K] - Gao Enming et al National Institute for the Control of Pharmaceutical and Biological Products, Bei jing
Media for growing, biochemical identifying and preserving as well as.pr otective agents for lyophi1ization of Neisseria gonorrhoeae were studied. The total 27 strains of N. gonorrhoeae tested were from both clinical collection and Center for Medical Culture Collection (CMCC). Results showed that all tested strains could grow well on both SPBA (special peptone blood agar) and PPBA (polypeptone blood agar) after 24 hours i ncubation. 26 out of 27 strains (about 96.3%) produced acid from glucose in NGCM-1 (N. gonorrhoeae carbohydrate medium-1). NGSM-1 (N. gonorrhoeae stron medium-1) could preserve N. gonorrhoeae for more than 30 days at 27℃. N. gonorrhoeae strains could be preserved for over 20 months by using de-fatted milk powder as protective agent during lyophilization.
1991 01 [Abstract][OnlineView][Download 177K]
1991 01 [Abstract][OnlineView][Download 42K] - Zhang Kun et al Xinjiang Institute for Endemic Disease Control and Research
The Treponema pallidum hemaggl utination test (TPHA) kit for syphilis using sonicated, purified T. pallidum (Nichols strain) antigen coupled to glutaraldehyde-stabilized sheep erythrocyte was prepared and compared with FTA-ABS and TP-ELISA. A total of 205 cases of syphilis was studied with 100% agreement between the TPHA and FTA-ABS. This kit was also tested with 920 non-syphilis sera, the false-positive rate was 1.0% for TPHA, and no false-positive for FTA-ABS.In conclusion, the TPHA was comparable in sensitivity and specificity to FTAABS test for syphilis and non-syphilis sera. Compared with the TP-ELISA and FTAABS, the TPHA was more easier and economical to perform.
1991 01 [Abstract][OnlineView][Download 173K]
1991 01 [Abstract][OnlineView][Download 45K] - Zhu Wei et al Shanghai Institute of Biological Products, Shanghai
An improved manufacturing process of FVⅢ concentrate was presented. By adding acid precipitation and ultrafiltration steps to the routine process, the product thus prepared had a much higher purity, and under the protection of suitable stabilizer could tolerate the severe dry heat-treatment at 80℃ for 72 hours. Results from three pilot production batches showed an average potency of 28.7±2.4 U/ml and a mean specific activity of 1.14 ± 0.11U/rag protein. The total recovery of activity from cryoprecipi tate extract was above60%. Preliminary clinical observation on three hemophilia A patients demonstrated the heat-treated product to be safe and effective with an average in vivo recovery of 98.1 ±31.7% and a half di sappearence time of 8 to 12 hours.
1991 01 [Abstract][OnlineView][Download 508K] - Wu Shaoyuan Chen Yan National Institute for the Control of Pharmaceutical and Biological Products, Beijing
It was found that the anti-HBs detection rate of RIA kit manufactured by NIVSB (National Institute of Vaccine and Serum, Beijing) was sometimes higher than that of Ausab (Abbott RIA kit). Neutralization test with plasma derived HBsAg or DNA recombinated HBsAg had been done and both antigens blocked the positive reactions. Mouse sera anti-normal human serum did not react with RIA kit of NIVSB or others. All these results confirmed high specificity and sensitivity of this RIA kit, which was agreeabie with the sensitivity test result by NICPBP. The NIVSB's kit could detect anti-HBs at a level of 0.5～2 mIU/ml while Ausab 1～5 mIU/ml.
1991 01 [Abstract][OnlineView][Download 136K] - Hong Hens et a l Shanghai Institute of Biological Products, Shanghai
An i mmunoblotting assay was established to detect the E. coli contam inants in the final products of reeombinant IFN. Antiserum against E. coli cell components was prepared by immunizing rabbits with lysate of E. coli harboring no human IFN gene. In order to get a satisfactory composition of antibodies against all of the E. coli cell components, antisera from four rabbits were pooled before use. When lysate of E. coli was separated by SDS-PAGE and then i mmunoblotted with the antiE. coli antiserum, all of its components, which were detectable by silver staining method, were also clearly shown in the result. Further study indicated that the immunoblotting method was not only highly, specific, but also more sensitive than silver staining method (SDS-PAGE) in detecting the E. coli contami nants. By using this method, no E. coli contaminant was found in the final products of human recombinant IFN-α_(?) prepared in our institute.
1991 01 [Abstract][OnlineView][Download 322K]
1991 01 [Abstract][OnlineView][Download 55K] - Wans Ya et al Shanghai Institute of Biological Products, Shanghai
The JEV multiplying in Vero cells in microcarrier system was studied by scanning and transmission electron microscopy. The virus was found in mitochondrion and endoplasmic reticulum. The size of the virus ranged from 30 to 46nm. The most abundant virus particles appeared 2～3 days after inoculation of virus.This is the morphological evidence showing that the JEV can multiply in the Vero cells in the microcarrier system.
1991 01 [Abstract][OnlineView][Download 890K]
1991 01 [Abstract][OnlineView][Download 85K] -
1991 01 [Abstract][OnlineView][Download 181K]
1991 01 [Abstract][OnlineView][Download 57K] -
1991 01 [Abstract][OnlineView][Download 39K] 下载本期数据