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1989 01 [Abstract][OnlineView][Download 341K]
1989 01 [Abstract][OnlineView][Download 40K] -
1989 01 [Abstract][OnlineView][Download 73K] - Li Junzhi Zhao Zhiwei Xu Deqi Changchun Institute of Biological Products
A part of htiman Cytomegalovirlus(HCMV)ADl69 EcoRI genomic frag- ments have been cloned in puc18 plasmid.The diaghostic assay for detecting HCMV DNA in urine,cord blood and GermicaI-vaginal secretions was developed by using 32 p-labelled cloned EcoRI-Z fragment of HCMV.The labelled probe can derect 12pg of HCMV DNA but fail to hybridize with 100ng of DNA from HSV_1,HS V2,HEL,λ phage or vector puc18.The HCMV DNA was detected from 4/50(8%)and 40/109(36%) urine specimens in normal newborn and newborn with Jaudice respectively.On the other hand,HCMV DNA were detected in 26/172(15%)white blood cells specimens which were randomly collected from cord blood.The presence of HC MV DNA in cervicitis and cervix cancer was found more frequently than in normal women.Dere- ction of HCMV by DNA hybridizaticn assay will be useful for rapid viral diagnosis with different clinical specimens.
1989 01 [Abstract][OnlineView][Download 581K] -
1989 01 [Abstract][OnlineView][Download 49K] - Jiang Jingwu Ji Songtao Wang Lixia National Serum and Vaccine Institute,Beijing
Some ctlturing conditions for hepatitis A virus were studied.Different days o1d FRhk-4 cell cuItures(a fetal rhesus monkey kidhey cell line)with different volumes of HAV were inoculated and observed.It Was found that hepatitis A virus didn’t shoW cytopathogenic effect of the cells and no viruses were detected in the supernatant by ELISA.The typical HAV Particles were found to be in the extract of the infected cell culture by immune electron microscopy.The typical perinuclear fine granular fluorescence of the cell infected with HAV Was seen by using immunofluores- cence staining.
1989 01 [Abstract][OnlineView][Download 519K] -
1989 01 [Abstract][OnlineView][Download 50K] - Dai Yongbiao Huang Peiren Shanghai Institute of Biological products
EIA was established to derect the pHSA-receptor titre of PreS2-HBsAg. The experimental results demonstrated that EIA had good specificity and reproducibil- ity.No significant difference Was found whether the pHSA was subjected to Sephadex G-200 chromatography or not.APPlying this method to detect the expressed products (PreS2-HBsAg pHSA-receptot)by recombinant Bombyx moti nucIear polyhydrosis virtts,both in cell culture supernatant and in ultrasonicated cell suspension,the expre- ssion 1eveI WaS higher in the latter.
1989 01 [Abstract][OnlineView][Download 128K] - Yuan Peina Liu Changnuan National Institute for the Control of Pharmaceutical and Biological Products
Two antigenic fractions are extracted from the cell wall of vibrio chole- rae El Tor belonging to serotypes,Inaba and Ogawa.They are present as soluble form in the media after lysis of microorganisms.These antigenic fractions are purified on a sephadex G-200 column.We have called antigenic fraction Ⅰ and Ⅱ,having molecular Wt.around 110,000 daltons and 60,000 daltons.The chemical structure of the fraction I consists of lypopolysaccharides,and the fraction Ⅱ is mainly proteins.Both two fra- ctions are antigens which induce the formation of specific antibody after immunization byeither parenteral or oral routes in rabbit or mice.The specific precipitation reaction of antigen-antibody is visualized in double agar gel diffusion.The vibriocidal and agglu- tination antibody titre is raised.The effective protection against challenge with viru- lent vibrio cholerae or enterotoxin in mice has been shown.Especially,after mixing fra- ction Ⅰ and Ⅱ,the immune response is more evident than single antigen fraction.When the fractions Ⅰ and Ⅱ were associated with the subunit B of cholera toxin,the heated toxin,or bacterial vaccine,these mixtures were respectively administed by oral in mice, their synergistic protection against challenge with the enterotoxin was different.The fraction Ⅰ and Ⅱ associated with whole bacterial vaccine could induce the most signifi- cant protection against challenge with the enterotoxin.By the immunodeficiency type- nude mice for the study of immunity to fraction Ⅰ and Ⅱ.We have found that both Ⅰ Ⅱ antignic fractions from cell wall of cholera vibrio and cholera toxin are two different lymphocyte dependent antigens,one is the B-cell dependent antigen,the other is the T-cell dependent antigen.So it is possible that two antigenic fractions will be developed into a important material of a new candidate vaccine of oral route.
1989 01 [Abstract][OnlineView][Download 606K] - Wang Shipeng Pseudomonas and Allied Bacteria Reference Laboratory of CMCCC(A) Chengdu Institute of Biological Products
Five strains of Pseudomonas aeraginosa untypable by 17 sera of IATS set were investigated by serological methods.All of the 5 strains except one,pA-87,that was of American origin,were clinical isolates from China.Results of cross-absorptions revealed that the.strains CMCC 10117,PA-87 and No.446 were new serotypes.We therefore sug- gest that it would be advantageous if these 3 strains could be included in IATS as ser- otypes 18、19 and 20 respectively. 465 of the 468 strains of Fseudomonas aeruginosa collected from various part(?) of China were proved to be typable with this new set of 20 sera by slide agglutination test,the typability being 99.4 per cent.
1989 01 [Abstract][OnlineView][Download 242K] - Hu Jiadi Changchun Institute of Biological products
Two strains of EHFV were studied by means of thin-section electron mic- roscopy.The results showed that the EHFV were adapted to growth and were sensible to GHK cells.The Morphology and morphogenesis of the two strains viruses are similar to that which was observed by Hung T.Three distinctive typs of viral inclusion bodies were identified also in EHFV infected GHK cells.the bodies have common morphological marker with that which was reported by Hung T.We observed ultrastructural changes of infected cells and dynamic circumstance of EHFV replication in the different days during infection process.
1989 01 [Abstract][OnlineView][Download 689K] - Ni Daoming National Vaccine and Serum Institute,Beijing Zhao Yanjun Chaoyang Hospital,Beijing Red Cross Society
A method for preparation of antithrombin Ⅲ(AT-Ⅲ)concentrated from human plasma has been described.After preliminary treatment of plasma with 10% dextran sulphate,MW 500,000,the isolation of AT-Ⅲ from the dextran sulphate supernatant was performed by affinity chromatography on heparin-sepharose at a ratio corresponding to 50 vol of plasma(batch washing and column elution);desalted by dialysis and concentrated. On disc gel electrophoresis,cellulose acetate strips electrophoresis and analysis of FPLC system,the AT-Ⅲ concentrates prepared showed single band or fraction.All the AT-Ⅲ samples traveled at the mobility of α2-globulins.Immunological specific activity of AT- Ⅲ was determined by radial immunodiffusion method. After immunization of rabbit with AT-Ⅲ antigen,the anti-AT Ⅲ serum with high titers and high specific activity was obtained.
1989 01 [Abstract][OnlineView][Download 513K] - Cheng Yaqin Hu Guozhen National Institute for the Control of Pharmaceutical and Biological Products
The first national reference for PKA(prekallikrein activitor)has been studied. The calibrations were performed with the first international standard for PKA(82/530) by means of a microtiter plate assay for PKA activity.
1989 01 [Abstract][OnlineView][Download 124K] - Liu Dezheng Zhou Baoming Li Wangchen National Vaccine and Serum Institute,Beijing
A purfied extract which containing two kinds of protective antigens Filamentous Hemagglutinin(FHA)and Pertussis toxin(PT)could be obtained from culture supern- atant of phase ⅠB.pertussis by a simple procedure using Blue-Sepharose Cl-6B chrom- atography.This extract possessed all biological activities of FHA and PT.The total amount of extracted antigens were found no difference between CS strain and Tohama strain,but the toxic activities were higher in Tohama strain than that of CS strain.The LPS was rare and agglutinogens could not be detected in the extract. This paper provided a useful method for the production of acellular pertussis vaccine.
1989 01 [Abstract][OnlineView][Download 533K] -
1989 01 [Abstract][OnlineView][Download 49K] - National Institute for the Control of Pharmaceutical and Biogical Products
We observed the reaction and the immune effect of anew formular for DPT vaccine with or without adsorption in field trial.The results showed that the immune effect of the adsorbed DPT was better than the non-adsorbed DPT and the reaction of the former was significantly lower than the latter.Such results provided the basis for revising requirement,unifying the formula and improving the quality of the vaccine. The sterile abscess rate was 0.29% throughout the observation.
1989 01 [Abstract][OnlineView][Download 222K] -
1989 01 [Abstract][OnlineView][Download 45K]
1989 01 [Abstract][OnlineView][Download 280K]